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The column oven now ensures, through its precise temperature control, that the temperature of the column is kept at a known and constant value. This makes the results comparable and reproducible.
Faster washing and sampling cycles
A higher column temperature reduces the viscosity of the sample or solvent, resulting in a significantly higher flow rate at constant pressure. As a result, both the sample and the washing cycles can be accelerated considerably.
As a result, significantly more samples can be measured in a given time period than with the same measurement at room temperature.
Allows the use of longer columns at constant pressure
Column length is often limited by the maximum pressure of the HPLC. If the viscosity of the sample or solvent decreases due to the higher column temperature, significantly longer columns can be used at constant pressure.